Fat body from short winged female cricket incapable of flight, indivd... - SRA

SRX272155: Fat body from short winged female cricket incapable of flight, indivdual 8
1 ILLUMINA (Illumina Genome Analyzer II) run: 3M spots, 226.5M bases, 127.4Mb downloads

Design: Poly(A) RNA was extracted directly from the tissues with the Micro-Poly(A)-Purist Kit (Ambion). mRNA was fragmented with fragmentation reagents (Ambion) at 70°C for 3 min 50 sec, column purified (RNA Clean & Concentrate kit-5, Zymo Research) and then subjected to first strand cDNA synthesis using oligo-dT, random hexamer primers and Superscript II reverse transcriptase (Life Technologies, Inc). Second strand cDNA was synthesized using RNaseH and DNA polymerse I (Epicentre). These cDNA fragments were column purified (DNA Clean & Concentrate Kit-5, Zymo Research) end-repaired (End-It DNA End Repair kit, Epicentre) and A-tailed using Klenow enzyme (NEB). An index adapter was ligated to the cDNA using Fast-Link DNA ligation kit (Epicentre). The library was size selected for 200-250 bp, enriched by PCR (Phusion DNA polymerase, Finnzymes), and purified using a PCR purification kit (Zymo Research) to create the final cDNA library. Tagged libraries were pooled in equal amounts and sequenced on an Illumina Genome analyzer II flow cell.

Submitted by: University of Nebraska-Lincoln

Study: Gryllus firmus Transcriptome or Gene expression

PRJNA198853 • SRP021536 • All experiments • All runs

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RNA-seq data of the wing polymorphic cricket Gryllus firmus.

Sample: Fat body from short winged female cricket incapable of flight, indivdual 8

SAMN02058422 • SRS416468 • All experiments • All runs

Organism: Gryllus firmus

Library:

Name: SW2_s_3_CGATGT

Instrument: Illumina Genome Analyzer II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 3M spots, 226.5M bases, 127.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR835513	2,980,677	226.5M	127.4Mb	2015-07-22

ID:: 381393

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